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Original Research Article | OPEN ACCESS

MicroRNA-542 suppressed the proliferation of human glioma cells by targeting talin-2 (TLN2)

Jinlan Li, Jiapeng Mo, Qunhui Liu, Wenjing Li, Shijun Yang, Minghui Tan, Jianhua Zhong

Department of Neurology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei 445000, China;

For correspondence:-  Jianhua Zhong   Email: 454175889@qq.com   Tel:+8613575656561

Accepted: 27 June 2022        Published: 31 July 2022

Citation: Li J, Mo J, Liu Q, Li W, Yang S, Tan M, et al. MicroRNA-542 suppressed the proliferation of human glioma cells by targeting talin-2 (TLN2). Trop J Pharm Res 2022; 21(7):1397-1402 doi: 10.4314/tjpr.v21i7.6

© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of miR-542 in the development of human glioma.
Methods: The expressions of miR-542 and TLN2 in glioma cells and normal human astrocytes were determined using qRT-PCR, while MTT and colony formation assays were used to determine cell proliferation. Western blotting was used to determine protein expression.
Results: It was revealed that miR-542 was significantly downregulated in glioma cells. Overexpression of miR-542 inhibited the proliferation and clonogenicity of glioma cells via induction of apoptosis. The percentage of apoptotic U87 cells increased from 5.32 in control to 26.76 upon miR-542 overexpression. Moreover, TLN2 was identified as the functional regulatory target of miR542 in glioma. The expression of TLN2 was markedly upregulated in human glioma cells. However, overexpression of miR-542 suppressed TLN2 expression. Silencing of TLN2 mimicked the tumor-suppressive effects of miR-542 in glioma cells, but this effect was blocked by TLN2 over-expression.
Conclusion: These results suggest that miR-542 exerted glioma-suppressive effect, with TLN2 as its functional regulatory target.

Keywords: Glioma, Proliferation, Micro-RNA, Tumorigenesis, MiR-542, Apoptosis, Prognosis, talin-2, Oncogene

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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